Enzyme Immobilization Kit
Combine your enzyme with our patented immobilization technology. Our evaluation kit includes six unique resin formulations and a titratable linker to maximize activity retention and stability.
- Dial in covalent attachment with our protocol
- Optimize stability with six resin formulations
- Works with your enzyme, no tag needed, purified or crude lysate
- Protocol, reagents, and our team’s support included
"The Cascade team has been very helpful and responsive during our enzyme immobilization tests. Our preliminary experiments already show improved catalytic activity for our trickiest enzyme using Cascade's products and we look forward to improving this further with the team's help!"
What's in the kit
A screening panel to identify the optimal immobilization for your enzyme and process.
- Six resin panel to quickly optimize process stability
- Titratable linker to optimize covalent attachment for maximum activity retention upon immobilization
- Step-by-step immobilization protocols, troubleshooting, SDS, and general guidance
- Buffers and reagents for a full screening panel
- Quick-start guide and direct access to our team
From your enzyme to an armored catalyst, in your lab
- BindMix your enzyme with the resins across the screening set.
- ScreenCompare activity and loading to find the best match.
- TestRun it under your real conditions: temp, solvent, pH.
- ReuseRecover the beads and run again. Count the extra batches.
Technical specifications
- Support material25-75 μm porous silica spheres
- Binding chemistryProprietary tunable covalent attachment
- Enzyme loading1-16% (w/w), depending on enzyme and immobilization conditions
- Solvent compatibilityResins compatible with aqueous and organic solvents; tolerance is enzyme-specific
- Thermal stabilizationTm increases of >10°C observed
- Storage stability6+ months dry, 1+ years at -20°C
- Scale-up particle sizes100-300 μm, 300-700 μm, and 1.5 mm silica beads
- Notable propertiesPorogen-free, low leaching, low non-specific adsorption, mechanically robust
Cascade has been a phenomenal team to work with, especially when I was troubleshooting experiments and addressing concerns related to the complex enzyme system I study. Their responses were always prompt, and the suggestions/support they provided were incredibly helpful.
The Cascade team has been very helpful and responsive during our enzyme immobilization tests. Our preliminary experiments already show improved catalytic activity for our trickiest enzyme using Cascade's products and we look forward to improving this further with the team's help!
We appreciate the quality products that Cascade Bio produces for us. Their materials have immobilized some of our enzymes with exceptionally well preserved activity and stability. Their expert support and convenient kits make immobilization testing straightforward.
Will it work with my enzyme?
Our approach is built to work across enzymes.
- Any enzyme class. 100% success across 40+ enzymes so far
- No exogenous tags necessary
- Purified enzyme or crude preparation
- Multimeric, cofactor-dependent, dynamic enzymes
Send us your enzyme and conditions. We'll advise before you buy.
Frequently asked questions
Tell us about your enzyme and your process. We'll get you a real answer, not a sales pitch.
What exactly do I get for $1,000?
One gram each of six unique enzyme immobilization resins, plus our proprietary linker for stable, tunable covalent attachment. This kit includes a thorough protocol, troubleshooting tips and tricks, a safety data sheet, buffers, reagents, and access to our immobilization experts. This price includes shipping & handling, and we typically ship within a week of receiving your order.
Do I need a polyhistidine-tag on my enzyme?
No. Our approach uses a proprietary titratable linker that can be titrated in to achieve the perfect amount of stable covalent attachment without activity loss. Non-covalent attachments, including affinity tags, tend to leach over time. Our covalent approach ensures high reusability of your enzyme with minimal leaching.
How long does a screening run take?
The kit includes a linker optimization step and a resin optimization step. Each takes about an hour of hands-on time plus an overnight incubation, so plan on two days. Shorter immobilizations are possible, with slightly reduced enzyme loading.
What happens after I find a resin that works?
We offer each resin in larger quantities and particle sizes to match your reactor. Contact us for a quote. We'll be your partners in getting your biocatalytic process to scale.
Do you ship internationally?
Yes. We’ve shipped our resins across four continents and counting.
See it work on your enzyme
The fastest way to know if our solution is right for you.
